RM
Keratinocyte, SIM micrograph
Keratinocyte. Structured illumination micrograph (SIM) of the intermediate filament network of a keratinocyte, as visualised with an antibody directed against the keratin 14 protein. Keratinocytes are the major type of skin cell, changing as they pass through the skin and eventually becoming dead keratinised cells at the skin's surface. This image was obtained using structured illumination microscopy, a technique that allows light microscopy to bypass the resolution limits imposed by the diffraction of light. This is OMX structured illumination microscopy, which uses 15 orientations and Fourier transform computer analysis.
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