Yeast cell nucleus, SIM micrograph
Yeast cell nucleus. Fluorescent structured illumination micrograph (SIM) of replication factories within a yeast cell nucleus. The colours represent: proliferating cell nuclear antigen (PCNA) protein (green), Nic96 nucleoporin protein of the nuclear pore complex (orange), and the spindle pole protein spc42 (orange dot, upper right). This image was obtained using structured illumination microscopy, a technique that allows light microscopy to bypass the resolution limits imposed by the diffraction of light. This is OMX structured illumination microscopy, which uses 15 orientations and Fourier transform computer analysis.
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